Happily, that's changed, and deservedly so. You don't see that many '60s Firebirds nowadays, not only due to their lower original volume but because enthusiasts long dismissed them as the Camaro-clones they appeared to be. That was still only a fraction of Camaro's business but roughly even with Cougar's -about what DeLorean had expected. Sales continued strong, with more than 100,000 units in 1968 and over 87,000 in '69. Engines were basically as before except that the Ram Air II gave way to a 345-bhp 400 Ram Air IV option. Unfortunately, the restyle also added somewhat to weight, as well as 2.3 inches in length and 1.3 inches in width. Notable was a more prominent chrome "bird beak" grille surrounded by trendy body-color Endura plastic à la GTO. The big news for '69 was the fire-breathing Trans Am, but all Firebirds sported new lower-body styling that further disguised their Camaro origins. At midyear, Pontiac offered a 400 Ram Air II option, also rated at 335 bhp, 25 below the comparable GTO unit due to a minor carb change. also became a 350 and claimed 35 more horses, the 400 tacked on five bhp, and a new 400 H.O. The ohc sixes grew to 250 cid, the 326 V-8 became a 350, the H.O. The '68s were little-changed visually but much-changed mechanically. About two-thirds carried optional power steering and Hydra-Matic Drive, suggesting Firebird competed less with Mustang - or Camaro - and more with Mercury's new '67 Cougar in an emerging "luxury ponycar" field. Though that was only half Camaro's volume, it pushed combined F-car sales well over the projected quarter-million breakeven point to the delight of GM accountants. With prices starting about $200 upstream of six-cylinder Camaros - $2,666 for the base hardtop and $2,903 for the convertible- the 1967-1969 Pontiac Firebird generated strong 82,000-plus sales despite an abbreviated debut season. 250 lively horses on regular gas" (and two-barrel carb). One step up was the V-8 Firebird 326, billed as a "family sportster. Next came the Sprint, with 215-bhp ohc six, floor shift, and "road hugging" suspension. The base Firebird carried Pontiac's year-old, 165-bhp 230-cid overhead-cam six. Each was available in Camaro's convertible and hardtop coupe body styles. Where Camaro achieved four models through options, Firebird arrived with five separate offerings keyed to engines. The most notable were engines set further back for better front/rear weight balance, and standard rear traction bars to minimize axle windup under hard acceleration.Īnother distinction involved marketing. Moreover, Pontiac's pony benefited from some engineering lessons learned too late to affect first-year Camaros, which went on sale some five months before Firebird's February 1967 debut. body had all the Chevrolet sheetmetal and all the same exterior hardware except for the grille and taillamps." Even so, those elements - split-theme grille, "slot" taillamps - were distinctly Pontiac, thus differentiating Firebird from Camaro to a surprising degree. Pontiac engineer Bill Collins later stated that the '67 Firebird was "just kind of inherited from Chevrolet." The. Firebird was a good name choice, signifying power, beauty, and youth in American Indian mythology and recalling GM's gas-turbine experimentals of the late '50s and early '60s. Pontiac was aware of the effort all along, and asked to be cut in once management vetoed DeLorean's two-seater. So DeLorean settled for a "Pontiacized" version of Chevy's four-seat Camaro - which was hardly bad.Ĭamaro stemmed from the "Panther" or "F-car" program that aimed at a direct Ford Mustang-fighter to replace the Corvair Monza as Chevy's mainstream sporty compact. But GM was hard-pressed to support one sports car, let alone two, and "Banshee" didn't test well with the public. Had division chief John DeLorean gotten his way, it would have been a two-seat sports car called Banshee, a low-cost sister to the Chevy Corvette. That was true even in the carefree '60s, when GM almost owned the market. General Motors may be the world's biggest automaker, but it's always had limits.
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The plugin will ask you to choose the desired postfix for all the names of processed images and result files, plus the directory where you want every resultant file to be saved.After you have completed the “Image straightening and ROI selection” steps, go to the plugins menu and select “ColonyArea” (Go to _Plugins -> ColonyArea -> Colony area).Thresholding and colony quantification using Colony_area: This option allows the user to analyze only a subsection of the plate if desired. The selection should be done in such a way that the sides of the rectangle touch the outer walls of the wells. (With an acceptable error of about 1-1.5 degrees max.) Then click on “OK”.Īfter the image has been straightened, use the “Rectangular Selection” tool from the ImageJ toolbar to make a selection of a region of interest (ROI) containing the wells you want to process (See Fig. Based on visual inspection, select an angle such that the image is nearly horizontal.1).įigure 1: Use the rotate command to straighten the image.įigure 2: Use the rectangular selection tool to choose the wells to be analyzed. Set the angle to 0, gridlines to anywhere from 20-100 depending on image sizeĪnd your discretion.Close all images opened in ImageJ and open the “.tiff” image of the colony formation assay that you want to process.Sample image files used in the manual can be downloaded here. Usageĭetailed usage instructions and examples here. In the Plugins dropdown of the Fiji menu, ColonyArea should now be available. Copy the following files to your Fiji plugins directory: Manual installĭownload the latest release from the repository. In the Fiji menu, go to Help -> Update… -> Manage update sites and select the ColonyArea site. InstallationĬolonyArea can be installed either through Fiji Update Sites or manually. The plugin processes each well individually and determines not the colony number, but the area of the well covered with cells, also taking the intensity into account. We have developed ColonyArea, an ImageJ-plugin that is optimized to perform standard analysis of colony formation assays conducted in 6- to 24-well dishes. These colony or focus formation assays are widely used in radiation biology and cancer biology, where they are employed to study resistance of cancer cells to radiation or the transforming potential of genes, respectively. We would really appreciate tips from more experienced users on how to improve our results.Camilo Guzmán 1,2, Manish Bagga 1,2, Amanpreet Kaur 1, Jukka Westermarck 1, and Daniel Abankwa 1ġTurku Bioscience, University of Turku, Åbo Akademi UniversityĬlonogenic assays measure the survival and growth of a single mammalian cell into a colony. It’s our first time trying to segment cells. Our best results are with automatic seeds and a radius of 25 px (that’s approx the size of a cell). For example, using the probability map from Weka Segmentation as seeds doesn’t seem to help. We have also tried playing around with the parameters for the watershed split. We tried retraining the algorithm in the Weka segmentation several times, but don’t know how to further improve the outcome. The issue is that we must keep the shape of our blobs with several cells stuck together for the watershed to be able to split them. I’m not sure Gaussian Blur is helping much since we must use very low sigma values (about 1). Erosion is definitely not helpful in our case.
In memory of Nichol Monique Howell, please visit our floral store. Talk of a Lifetime Why Plan Ahead Pre-Planning Form Plan A Funeral. Serenity-Simmons Funeral Home of New Albany is in charge of the arrangements. About Us Our Location Our Calendar Pre-Planning. Visitation will be held on Saturday, Decemfrom 3-6p.m., at Serenity-Simmons Funeral Home Chapel in New Albany. Calling hours are 1:00pm-6:00pm Sunday, Apat Serenity Funeral Chapel, 2340 W. She was preceded in death by her mother Etheleen Howell, grandparents Mildred and Charles Christian, aunts Barbara Williams and Margie Henry. A host of cousins, friends and other relatives. Louis, MO, Deborah Jones of New Albany, MS and a special friend Steve Chandler of Dorsey, MS. She leaves to cherish her loving memories two sons: Steve Howell and Dewaughn Howell of Doresy, MS, her father Dwayne Howell, two brothers: Dwayne Howell ll and Walter (Aquarium) Howell of Jackson, MS, nieces Nazaria Howell and Melody Clark and Chayce Clark of Jackson, MS, uncles Charles (Beatrice) Hinkle of Flint, MI, Gary (Stacie) Christian of New Albany, MS, James Hinkle of Flint, MI, aunts Pearlie Traylor, Theresa Hinkle and Melba Hinkle all of Flint, MI, Ruby Hinkle of St. About Us Our Location Our Calendar Pre-Planning. Calling hours are 1:00pm-6:00pm Monday, Januat Serenity. Funeral services will be held 11:00am Tuesday, Januwith a 10:30am family hour at Serenity Funeral Chapel, 2340 W. Call: 81 Menu Call: 81 Directions Facebook Home Obituaries Who We Are. Alonzo-age 77, of Flint, passed away Friday, Januat Willowbrook Manor. Read Serenity Funeral Chapel obituaries, find service information, send sympathy gifts, or plan and price a funeral in Flint, MI. Nicole loved spending time with family and friends, talking on the phone, singing, shopping and watching movies. View Recent Obituaries for Serenity Funeral Chapel. She attended Flint Community Schools in her younger years and later furthered her education in the Itawamba County School District in Fulton, MS. Banks Funeral Chapel 'Your Choice for Compassionate Care' Proudly serving the Flint, Michigan area. Nicole accepted Christ at an early age at Gospel Temple of Flint, MI. She was born on Jin Flint, MI, to the late Etheleen Howell and Dwayne Howell. Calling hours are 1:00pm-6:00pm Monday, Januat. Funeral services will be held 11:00am Saturday, Decemwith a 10:30am family hour at Serenity Funeral Chapel, 2340 W. Funeral services will be held 11:00am Tuesday, Januwith a 10:30am family hour at Serenity Funeral Chapel, 2340 W. It is always difficult saying goodbye to someone we love and cherish. Mario-age 45, of Flint, passed away Friday, Decemat McLaren Flint. Nichol Howell, 42, transitioned on Wednesday, Decemat North Mississippi Medical Center in Tupelo. Alonzo-age 77, of Flint, passed away Friday, Januat Willowbrook Manor. This completes the basic setup for the project. We can change the label of this pin by right clicking on the PC13 Pin and selecting Enter User Label option. Hence, zoom into the Pinout view of the STM32F103C8T6 MCU and on the PC13 Pin, make a left click and select GPIO_Output option. We know from the schematic of the STM32 Blue Pill that a Green LED is connected to PC13. The next step is to configure the LED present on the Blue Pill Board. As soon as you select this option, you can observe that the Pins PA13 and PA14 are SWDIO and CK i.e. In the Pinout and Configuration Tab, expand the System Core block and select the SYS option. The main project window will open with a pinout of STM32F103C8T6 MCU on the right and configuration options on the left. Select that MCU and on the top right corner click on the Start Project option. On the right side, our MCU will be listed. In the new window, under the MCU/MPU Selector tab, search for “STM32F103C8”. A new window will open for us to select the MCU that we are working on. Now, in the main window, select the ACCESS TO MCU SELECTOR option. After completion, you can close this window. It will download and install the MCU Package. Select the latest version and click on Install Now option at the bottom. In that, select the STM32Cube MCU Packages option and expand the STM32F1 tab (since our MCU is STM32F103C8T6). On the right side in the application, click on the install or remove embedded software packages option. At the time of writing this tutorial, the version of STM32CubeMX is 5.6.1. You have to login to ST in order to download the software. Download this software from this official STMicroelectronics website. Download and Install STM32CubeMXįirst, let us start with the STM32CubeMX Code Generator Tool. I will show you how to use all these software and hardware so that you can easily program STM32F103C8T6 using Keil uVision and STM32CubeMX. This is also provided by STMicroelectronics and is used to generate the code for a specific STM32 MCU as well as a particular IDE. There is another important software called the STM32CubeMX. Although the original programmer will be expensive, there are many third-party programmers that are compatible with the original programmer and cost very less. It is as in-circuit programmer and debugger developed by STMicroelectronics. This is a great tool for working with ARM based MCUs like our STM32F103C8T6 and it also supports proper debugging.Ĭoming to the hardware, we have to use a separate hardware for programming (as well as debugging) the STM32 Blue Pill Board. Speaking of software, I suggest you to use the ARM’s Keil uVision IDE. The proper way is to step away from the Arduino world and enter a more professional set of tools in terms of both the software as well as the hardware. This is acceptable for beginners but if you want to seriously learn the working of the powerful STM32 MCU, then this is not the right way. Using Arduino IDE for STM32 projects is very easy as the familiar Arduino terminology can be applied to STM32F103C8T6 as well. In all these projects, I have used the Arduino IDE as the tool for writing the code as well as uploading the code to STM32 using an USB to UART Converter. Till now, I have implemented several STM3F103C8T6 MCU based projects using the STM32 Blue Pill Board. You will learn how to install the Software (both Keil and STM32CubeMX), configure the software as per our requirements and upload the code using ST-Link Debugger and Programmer. In this tutorial, I will show you How to Program STM32F103C8T6 using Keil uVision and STM32CubeMX Code Generator Software. “You may buy HUD property with an FHA loan. “The cons are most are not in good condition and will need additional rehabbing before a buyer can move into them,” she said. FHA loans can be used to purchase HUD homes and may help cover the cost of a rehab. Robinson noted that they are sold below market price, but that the condition of the properties can be a problem for buyers. HUD itself sells one- to four-unit residential properties that the agency has taken in foreclosure on FHA-insured mortgages. By protecting lenders against losses, HUD enables home purchases by buyers who may have poor credit or who might not be able to put up a large down payment. Established as part of the Federal Housing Act of 1934, the FHA has since insured more than 50 million mortgages on single-family homes, multiunit buildings and even hospitals. The FHA is the branch of HUD that is most closely associated with encouraging home ownership and increasing the number of available homes. HUD’s programs cover Section 8 housing, community development grants, homebuyer assistance, home rehab work, homeless assistance and combating housing discrimination. HUD consolidated five federal housing and development agencies: the Federal Housing Administration, the Public Housing Administration, the Federal National Mortgage Association (Fannie Mae), the Urban Renewal Administration and the Community Facilities Administration. The legislation enabled the federal government to coordinate efforts to solve the problem of substandard and deteriorating housing. HUD was created by the landmark Department of Housing and Urban Development Act of 1965 as a means of addressing housing inequality in U.S. “Because the homes are sold at a discount, it helps revitalize the areas, keeping vacant homes occupied as many (HUD homes) are vacant.” What is HUD? “They also are sold under the Good Neighbor Next Door program for police officers and other government officials in underserved neighborhoods,” Robinson said. While HUD REO (bank-owned) homes are typically sold online through the HUDHomes website, she explained, sometimes they are listed in the MLS for agents to share with their buyers. It’s essential, then, that agents know how to navigate the paperwork and bureaucracy of a HUD transaction, or know where to get help.Īndretta Robinson, team lead for the Titan Group powered by RE/MAX 10, has experience helping clients purchase homes through HUD. The same is true of agents whose portfolios include homes that were built with federal funding. Fair housing opportunities, neighborhood development and improvement all fall under HUD’s purview.Īny agent who works with a client who has procured Fannie Mae or Freddie Mac loans is effectively working for a HUD client. The United States Department of Housing and Urban Development (HUD) generally remains in the background, despite being the principal agency tasked with administering the nation’s housing needs. Brokers and agents must spread the word that mortgages are available at 4.5% if you apply.When government assistance enters the homeownership conversation, the subject often centers on federal lending giants Fannie Mae and Freddie Mac. For those who haven’t drowned their credit scores, home purchasing conditions are very advantageous. For qualifying homeowners, a Home Protect warranty can also provide discounts of up to 30% on appliance purchases.įirst tuesday take: Buyer’s agents can take advantage of Freddie Mac’s effort to move its foreclosure REO inventory by investigating whether their buyer qualifies for incentive programs such as these.Ĭalifornia’s excessive supply of real estate owned (REO) properties will continue to bulge until buyers regain purchasing confidence. Some HomeSteps REO properties may be eligible for a two-year Home Protect warranty covering electrical, plumbing, air conditioning, heating and other appliances. Purchase agreement offers must be received by Jand escrow must close on or before September 30, 2011. Freddie Mac is offering up to 3.5% in closing cost assistance and a $1,200 buyer’s agent bonus for HomeSteps real estate owned (REO) properties sold to owner-occupants. If you are looking for ReiBoot for Android alternatives, we recommend you to download EaseUS MobiSaver for Android or iSkysoft Toolbox for Android. ReiBoot for Android is 100% FREE and can be used without restrictions on any desktop and laptop PC running modern versions of Windows OS such as Windows 7, 8, 10 (both 32-bit and 64-bit) and the new Windows 11. While ReiBoot for Android is not a comprehensive Android smartphone and tablet recovery utility, it can help countless users who are experiencing freezes at boot and the inability to reach certain important parts of the boot sequence. It is important to note that this application supports only these 1-click operations, and does not allow users to manually snoop in the critical OS/kernel data partitions, redacting the potential for damaging the OS and making the Android device unstable or inaccessible for future use. The app will notify users when the Android device is connected and will even try to identify it if it can. Before trying to use any of these tools, users are required to connect their Android device to the PC via USB cable. They include one-click buttons for Entering Fastboot Mode, Exiting Fast Boot Mode, Enter Recovery Mode, Exit Recovery Mode, Enter Download mode (Odin mode for transferring data to and from the device), Exit Download Mode, and Clear System Cache. ReiBoot for Android features a very streamlined user interface with a single dashboard that offers a listing of all available tools.
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